Potency data for probe A-079 and control A-226
No in vitro potency data available.
In cell potency
| Target name | Target information | Species | Assay description | Compound conc. | A-079 | A-226 | Publication of assay conditions |
|---|---|---|---|---|---|---|---|
| TRPA1 | recombinant protein expressed in cells; full length | Homo sapiens | Fluorometric imaging plate reader (FLIPR)-based Ca2+ assay: AITC (allylthiocyanate) evoked robust increase of intracellular Ca2+ was blocked. | 0.03-0.3 µM | IC50 = 0.067 µM ± 0.004 µM (n=8) | IC50 > 100 µM | PMID: 21402443 |
| TRPA1 | HEK-293F cells transiently overexpressing hTRPA1; full length | Homo sapiens | Electrophysiological assay: Blocking of AITC ( allylthiocyanate, 100 µmol/L)-evoked currents: HEK-293F cells were held at -60 mV | IC50 = 51 nM | PMID: 21402443 |