Potency data for probe BAY-3827 and control BAY-974
In vitro potency
| Target name | Target information | Species | Assay description | Compound conc. | BAY-3827 | BAY-974 | Publication of assay conditions |
|---|---|---|---|---|---|---|---|
| PRKAA1 | Homo sapiens | Biochemical PRKAA1 assay with 10 µM ATP (Bayer in house) | IC50 = 1.4 nM | IC50 > 20 µM | |||
| PRKAA1 | Homo sapiens | Biochemical PRKAA1 assay with 2 mM ATP (Bayer in house) | IC50 = 15 nM | IC50 > 20 µM | |||
| PRKAA1 | Homo sapiens | Eurofins kinase panel (PRKAA1) | 1 µM | IC50 = 7 nM | |||
| RPS6KA1 | Homo sapiens | Eurofins kinase panel (RPS6KA1) | 1 µM | IC50 = 9.1 nM |
In cell potency
| Target name | Target information | Species | Assay description | BAY-3827 | BAY-974 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| PRKAA1 | Homo sapiens | Cellular mechanistic pACC assay using IMR-32 neuroblastoma cells : Quantification of ACC phosphorylated at Ser79 (pACC) in cell lysates | IC50 = 150 nM | IC50 > 30 µM | ||
| PRKAA1 | Homo sapiens | Cellular mechanistic pACC assay using Colo-320 cells : Quantification of ACC phosphorylated at Ser79 (pACC) in cell lysates | IC50 = 390 nM | IC50 > 30 µM |