Potency data for probe THNAN69 and control THNAN69-NC
In vitro potency
| Target name | Target information | Species | Assay description | THNAN69 | THNAN69-NC | Publication of assay conditions |
|---|---|---|---|---|---|---|
| LIMK1 | Homo sapiens | DSF (LIMK1) | deltaTM = 6.9 °C | |||
| LIMK1 | Homo sapiens | SPR (LIMK1) | KD = 249 nM |
In cell potency
| Target name | Target information | Species | Assay description | Compound conc. | THNAN69 | THNAN69-NC | Publication of assay conditions |
|---|---|---|---|---|---|---|---|
| LIMK2 | HEK293 cells | Homo sapiens | NanoBRET (LIMK2) | EC50 = 80 nM | |||
| LIMK2 | HuCCT1 cells (human intrahepatic cholangiocarcinoma; male; ATCC, CRL-1997) | Homo sapiens | EGFP-LIMK2 depletion assay (LIMK2) | DC50 = 1 nM | |||
| LIMK2 | HuCCT1 cells (human intrahepatic cholangiocarcinoma; male; ATCC, CRL-1997) | Homo sapiens | LIMK2 degradation | 10 nM | Dmax ~ 90% | Dmax = 0% | |
| LIMK1 | HEK293 cells | Homo sapiens | NanoBRET (LIMK1) | EC50 = 412 nM |