In cells

Use probe and control at concentration up to 1 µM.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Not recommened for in vivo use. A-1596856 shows poor passive permeability and low in vitro hepatocyte stability which led to in vivo high clearance, low half life and low oral bioavailability in a rat in vivo pharmacokinetics study.