In cells

Effective range is up to 100 nM but higher concentrations can be used without introduction of off-target effects.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Endothelin-1-induced pressor response was dose-dependently blocked in conscious rats by a sinle oral dose (1 - 100 mg/kg).