In cells

Use at a concentration between 1-10 µM. We recommend to perform a dose dependent degradation assay as the appropriate concentration for the probe can be varying according to the cell line and its genomic background.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Tested with 30 mg/kg s.c. q.d .in mice; results in KRAS degradation. GP2d KRAS G12D and RKN KRAS G12V mutant tumours shrink upon ACBI3 treatment without significant body weight change.