In cells

Use at concentrations up to 1 µM. We recommend to test at various concentrations with a 9 point curve starting from 1 µM down in 1/3 serial dilutions.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Suitable for in vivo studies: Tested in mice with 10 mg/kg, IP administration. GSK778 was well tolerated and showed a good exposure.