Usage recommendations for probe PF-04457845 and control PF-04875474
In cells
Use at concentration of 0.2 to 1 µM.
Note FAAH inhibition is time-dependent.
Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.
In vivo
Anti-hyperalgesic activity at 1-3 mg/kg p.o. in rats.