Usage recommendations for probe THPP-1 and control THPP-1-NC
In cells
Recommended concentration ~ 100 nM
We recommend to use the PDE10A inhibitor JNJ-42396302 also available from SGC for control experiments.
Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.
In vivo
THPP-1 has a high passive permeability and a good oral bioavailability. Use between 1 and 10 mg/kg PO. The probe was tested in rats with up to 50 mg/kg PO.