Potency data for probe BI-1915 and control BI-1920
In vitro potency
| Target name | Target information | Species | Assay description | BI-1915 | BI-1920 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| CTSS | Homo sapiens | Scintillation proximity assay (CTSS) | Kd = 31 nM | Kd = 272 nM | ||
| CTSS | Homo sapiens | Luminescent based enzymatic assay (CTSS) | IC50 = 17 nM | IC50 > 20 µM | PMID: 23084902 (supplement) | |
| CTSK | Homo sapiens | Luminescent based enzymatic assay (CTSK) | IC50 > 10 µM | |||
| CTSL | Homo sapiens | Luminescent based enzymatic assay (CTSL) | IC50 > 30 µM | PMID: 23084902 (supplement) | ||
| CTSB | Homo sapiens | Luminescent based enzymatic assay (CTSB) | IC50 > 10 µM |
In cell potency
| Target name | Target information | Species | Assay description | BI-1915 | BI-1920 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| CTSS | Homo sapiens | Antigen challenge cell assay: Based on the use of B cells to “present” exogenous antigen (added ovalbumin) via a cathepsin S dependent process to T cells. | IC50 = 2.8 nM | PMID: 23084902 (supplement) |