Potency data for probe BI 207127 and control BI-7656
In vitro potency
| Target name | Target information | Species | Assay description | BI 207127 | BI-7656 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| HCV NS5B | Hepacivirus C | Enzymatic assay with HCV NS5B polymerase D21: SPA measuring 3H-UTP incorporation | IC50 = 19 nM | IC50 > 5 µM |
In cell potency
| Target name | Target information | Species | Assay description | BI 207127 | BI-7656 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| HCV NS5B | Huh7 cells | Hepacivirus C | Cell based replicon assay, genotype 1a: Replicon assay using RT PCR for RNA quantification, 72 h incubation | EC50 = 23 nM | ||
| HCV NS5B | Huh7 cells | Hepacivirus C | Cell based replicon assay, genotype 1b: Replicon assay using RT PCR for RNA quantification, 72 h incubation | EC50 = 11 nM |