Potency data for probe BI-2540 and control BI-2439
In vitro potency
| Target name | Target information | Species | Assay description | BI-2540 | BI-2439 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| HIV NNRT | Human immunodeficiency virus 1 | HIV1 reverse transcriptase picogreen fluorescence assay (enzymatic): 15 min pre incubation before adding substrate solution, 50 min incubation at 37° C, PicoGreen as a fluorescent intercalator. | IC50 = 12 nM | IC50 = 5053 nM |
In cell potency
| Target name | Target information | Species | Assay description | BI-2540 | BI-2439 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| HIV NNRT | C8166 cells | Human immunodeficiency virus 1 | HIV viral replication assay (ELISA): 72 h incubation at 37°C, readout: ELISA quantification of p24 | IC50 = 0.76 nM | ||
| HIV NNRT | C8166 cells | Human immunodeficiency virus 1 | HIV 1 luciferase assay: 72 h incubation at 37°C, readout: RLU of luciferase, Steady Glo | IC50 = 2.6 nM |