Potency data for probe BI 99179 and control BI 99990
In vitro potency
| Target name | Target information | Species | Assay description | BI 99179 | BI 99990 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| FASN | Human FAS enzyme isolated from HeLa cells | Homo sapiens | Human FAS enzyme assay: Measurement of the decrease in fluorescence due to the formation of NADP from NADPH in the reductive steps. | IC50 = 79 nM | IC50 > 3 µM | PMID: 21873051 |
In cell potency
| Target name | Target information | Species | Assay description | BI 99179 | BI 99990 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| FASN | Mus musculus | Inhibition of [14C]acetate incorporation into mouse N-42 cells | IC50 = 0.57 µM | PMID: 21873051 | ||
| FASN | Homo sapiens | Inhibition of [14C]acetate incorporation into human H1975 cells | IC50 = 0.18 µM |