Potency data for probe FHT-2344 and control FHT-5908
In vitro potency
| Target name | Target information | Species | Assay description | FHT-2344 | FHT-5908 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| SMARCA2 | Homo sapiens | Measurement of the ATPase catalytic activity using the ADP-Glo detection (SMARCA2) | IC50 = 13.8 nM ± 3.300 nM (n=8) | IC50 = 1.88 µM ± 0.320 µM (n=5) | ||
| SMARCA4 | Homo sapiens | Measurement of the ATPase catalytic activity using the ADP-Glo detection (SMARCA4) | IC50 = 26.1 nM ± 7.300 nM (n=8) | IC50 = 3.99 µM ± 0.680 µM (n=5) | ||
| CDH4 | Homo sapiens | Measurement of the ATPase catalytic activity using the ADP-Glo detection (CDH4) | IC50 > 200 µM (n=4) |
In cell potency
| Target name | Target information | Species | Assay description | FHT-2344 | FHT-5908 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| SMARCA2 | Homo sapiens | Luciferase reporter assay: SMARCA2 transcriptional activity in a SMARCA4-mutant cell line | IC50 = 29.8 nM ± 13.400 nM (n=7) | IC50 = 4.35 µM ± 1.290 µM (n=3) | ||
| SMARCA4 | Homo sapiens | Luciferase reporter assay: SMARCA4 transcriptional activity in a SMARCA2-mutant cell line | IC50 = 30.2 nM ± 8.600 nM (n=6) | IC50 = 2.37 µM ± 0.850 µM (n=3) |