Potency data for probe JNJ-65355394 and control JNJ-73924149
In vitro potency
| Target name | Target information | Species | Assay description | JNJ-65355394 | JNJ-73924149 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| OGA | Recombinant human protein expressed in HEK293T cells; full-length | Homo sapiens | Fluorescence-based enzymatic assay: Meassured the hydrolysis of fluorescein mono-beta-D-N5 Acetyl-Glucosamine (FM-GlcNAc) | IC50 = 1.3 nM | IC50 > 10 µM | PMID: 26567273 |
In cell potency
| Target name | Target information | Species | Assay description | JNJ-65355394 | JNJ-73924149 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| OGA | Homo sapiens | Cell-based OGA assay: High content screening (Perkin Elmer opera phenix) using a monoclonal antibody detecting all GlcNAcylated protein levels (CTD110.6 Cell signaling 9875) in HEK cells overexpressing P301L mutant human Tau: | IC50 = 3.9 nM | IC50 > 1 µM |