Potency data for probe SGK3-PROTAC1 and control cisSGK3-PROTAC1
In vitro potency
| Target name | Target information | Species | Assay description | SGK3-PROTAC1 | cisSGK3-PROTAC1 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| SGK3 | Homo sapiens | SGK3 radioactive filter binding assay using 33P ATP (Dundee kinase screen) | IC50 = 300 nM | IC50 = 600 nM | PMID: 31461270 |
In cell potency
| Target name | Target information | Species | Assay description | Compound conc. | SGK3-PROTAC1 | cisSGK3-PROTAC1 | Publication of assay conditions |
|---|---|---|---|---|---|---|---|
| SGK3 | HEK293 cells | Homo sapiens | SGK3 degradation assay: 48 h incubation, no effect on RPS6KB1, SGK1 and SGK2 | 100 nM | degradation = 65% | degradation = 0% | PMID: 31461270 |
| RPS6KB1 | HEK293 cells | Homo sapiens | RPS6KB1 degradation assay | degradation = 0% | PMID: 31461270 | ||
| SGK1 | HEK293 cells | Homo sapiens | SGK1 degradation assay | degradation = 0% | PMID: 31461270 |