Potency data for probe TP-004 and control TPn-004
In vitro potency
| Target name | Target information | Species | Assay description | TP-004 | TPn-004 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| METAP2, KAT6A | Homo sapiens | Biochemical activity-MetAP2 using Co | IC50 = 31 nM | PMID: 22086725 | ||
| METAP2, KAT6A | Homo sapiens | Biochemical activity-MetAP2 using Mn | IC50 = 7 nM | PMID: 30069049 | ||
| EDNRA, METAP2 | Membranes prepared from CHO cells stably transfected with human EDNRA and EDNRB | Homo sapiens | Inhibition of [125I]-endothelin-1 binding to EDNRA | IC50 > 10 µM | PMID: 10082200 |
In cell potency
| Target name | Target information | Species | Assay description | TP-004 | TPn-004 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| METAP2 | HUVEC cells (Lonza) | Homo sapiens | Cellular assay: Cell activity Western Blot Nmet14-3-3gamma HUVEC: Measurement of the accumulation of N-Met 14-3-3gamma which is a client protein of MetAP2 | EC50 = 15 nM | PMID: 27155900 |