In cells

Use at concentration from 0.1 to 1 µM

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Efficacy in mechanistic mouse model in vivo (CFA Model): 0, 12.5, 25, 50 mg/kg, p.o.; 1 single dose at 1h before PGE2 sampling
Efficacy in PD pain mouse model in vivo: 50 mg/kg, p.o.; 1 single dose each day at 1h before DWB; DWB testing at 24 and 48 h after CFA (Vehicle : EtOH/Solutol/NaCl 1:5:94)