In cells

Use at concentration of 300 nM.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Tested in mice, rat and dog at 0.5 to 5 mg/kg (po) and 0.5 to 1 mg/kg (iv); Orally bioavailable. 2-week Repeat-Dose Rat Toxicity Study with up to 100 mg/kg per day: No clinical signs, clean toxicity profile.
Strong inhibition of histone H2A-Thr120 phosphorylation in HeLa-MaTu cervical ca xenograft on nude mice.