In cells

Use at a concentration up to 300 nM.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

For in vivo studies in rat, use a maximum dose of 6 mg/kg. This dosage effectively covers the IC₅₀ and is well tolerated for up to 7 days. However, extending the dosing period beyond 7 days lead to increased mortality rates in rodents for reasons that remain unresolved. Oral administration of BAY-439 (2-days QD treatment) reduced inflammatory pain in the complete Freund’s adjuvant (CFA)-induced arthritic rat model rat.