In cells

Due to the good cellular permeability use BI-1230 in concentration < 100 nM. Note: The cellular permeability of the control BI-1675 was not determined and BI-1675 was not tested in the cell-based HCVPV RNA replication Luciferase reporter assay, so its utility as control in other than the in vitro protease assay is unknown.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

BI-1230 was tested in rat, dog or cynomolgus monkey at 2 mg/kg iv or 5 mg/kg po.