In cells

Use at a concentration of 100 nM.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Tested in vivo in rats (i.v. and p.o. dose 4.5 mg/kg) and mice (i.v. dose 4.5 mg/kg, p.o. dose 2 mg/kg), suggested is oral dose. Oral administration of the compound at 0.1, 0.3 and 1mg/kg dose-dependently reduced premature responses in the 5CSRTT (5-choice serial reaction time impulsivity task) in Lister Hooded rats (associated calculated free brain exposures were 8, 18 and 181 nM). Rats were trained to perform the task and on the day of the experiment were tested at 1 hour post drug treatment.