In cells

Use at a concentration up to 10 µM.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Tested in vivo: i.p. dosing typically used in mouse; suitable for oral dosing in rat, dog; Intraperitoneal administration (30 mg/kg) inhibited [3H] SAR-127303 binding in the rat hippocampus, indicating brain penetration. Mouse dose-response study: The measured 50% effective dose (ED50) was 0.5 mg/kg (95% confidence interval, 0.4–0.7 mg/kg) (calculated plasma exposure: 51 ng/ml, brain exposure: 30 ng/g).Prolongs wakefulness in rats (30 mg/kg).