Usage recommendations for probe JNJ-8003 and control JNJ-6643
In cells
Use at a concentration of 1 - 10 µM.
Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.
In vivo
Tested in mice with up 100 mg/kg (po dose in PEG-400); Shows statistically significant and dose dependent reduction of viral load and viral RNA when tested in Balb-C mice. Tested in neonatal lambs with 0.25 to 4 mg/kg in PEG-400 orally: As from 1 mg/kg lungs are completely preserved from lesion formation.