In cells

Use at a concentration of 100 nM.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Tested in mice with oral administration. FAK phosphorylation inhibition (50%) was sustained in vivo for over 8 hours with a single oral dose of 30 mg/kg. No weight loss, morbidity or mortality was observed in any tumor growth inhibition experiment (up to 50 mg/kg BID x 17 days).