In cells

Use at concentration from 1 to 10 μM.

Cytotoxicity assay for TP-020 using HepG2 cell [% of ATP content relative to that of untreated cells]: for 10 μM Cytotoxicity = 97; 30 μM Cytotoxicity = 87

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

TP-020 effectively and dose-dependently suppressed the elevation of plasma TG level after oral administration at 1mg/kg and 3mg/kg. Five-week repeated administration of TP-020 significantly prevented body weight gain and fat accumulation in mice fed HFD.