Potency data for probe 8RK64 and control JYQ88
In vitro potency
| Target name | Target information | Species | Assay description | 8RK64 | JYQ88 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| UCHL1 | recombinant human protein | Homo sapiens | UCHL1 inhibition assay with Ub-Rho-morpholine as substrate | IC50 = 320 nM | IC50 = 12.9 µM | PMID: 32886496 |
| UCHL3 | Homo sapiens | UCHL3 inhibition assay with Ub-Rho-morpholine as substrate | IC50 = 216 µM | PMID: 32886496 | ||
| UCHL5 | Homo sapiens | UCHL5 inhibition assay with Ub-Rho-morpholine as substrate | IC50 >> 200 µM | PMID: 32886496 |
In cell potency
| Target name | Target information | Species | Assay description | 8RK64 | JYQ88 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| UCHL1 | HEK293 cells | Homo sapiens | UCHL1 activity based protein profiling assay (ABPP): 1h, 37°C | Full inhibition = 3 µM | PMID: 32886496 |