Potency data for probe BAY-474 and control BAY-827
In vitro potency
| Target name | Target information | Species | Assay description | BAY-474 | BAY-827 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| MET | Recombinant human MET kinase enzyme domain | Homo sapiens | Homogeneous Time Resolved Fluorescence Assay: Quantification of enzymatic phosphorylation of biotinylated heterocopolymeric polypeptide using HTRF assay technology | IC50 < 1 nM | IC50 = 11 µM |
In cell potency
| Target name | Target information | Species | Assay description | BAY-474 | BAY-827 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| MET | Homo sapiens | Cellular mechanistic assay using NKM-45 cells and p-MET | IC50 = 2.9 nM | |||
| Homo sapiens | Cell proliferation with Hs746T cells | IC90 = 56 nM | ||||
| Homo sapiens | Cell proliferation with NKM-45 cell line | IC90 = 24 nM |