In cells

Use between 1 nM to 1 µM, not higher than 10 µM.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

BAY-474 shows a suitable pharmacokinetic profile for in vivo studies and was effective in a large tumor model in nude mice with 10 mg/kg BID orally.