Potency data for probe BAY-985 and control BAY-440
In vitro potency
| Target name | Target information | Species | Assay description | BAY-985 | BAY-440 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| TBK1 | Homo sapiens | Biochemical assay (low ATP) with TBK1 | IC50 = 2 nM | IC50 = 1140 nM | ||
| TBK1 | Homo sapiens | Biochemical assay (high ATP) with TBK1 | IC50 = 18 nM | IC50 > 20 µM | ||
| IKBKE | Homo sapiens | Biochemical assay (low ATP) with IKBKE | IC50 = 2 nM | IC50 = 1280 nM |
In cell potency
| Target name | Target information | Species | Assay description | BAY-985 | BAY-440 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| TBK1 | Homo sapiens | NanoBRET with TBK1 (SGC Frankfurt) | EC50 = 48 nM ± 5.100 nM (n=3) | EC50 > 50 µM (n=3) | ||
| IKBKE | Homo sapiens | NanoBRET with IKBKE (SGC Frankfurt) | EC50 = 420 nM ± 210.000 nM (n=3) | EC50 > 50 µM (n=3) | ||
| MAP3K19 | Homo sapiens | NanoBRET with MAP3K19 (SGC Frankfurt) | EC50 > 50 µM (n=3) | EC50 > 50 µM (n=3) | ||
| STK17A | Homo sapiens | NanoBRET with STK17A (SGC Frankfurt) | EC50 = 4.8 µM ± 2.000 µM (n=3) | EC50 = 5.1 µM ± 2.000 µM (n=3) | ||
| MAP2K5 | Homo sapiens | NanoBRET with MAP2K5 (SGC Frankfurt) | EC50 > 50 µM (n=3) | EC50 > 50 µM (n=3) | ||
| FLT3 | Homo sapiens | NanoBRET with FLT3 (SGC Frankfurt) | EC50 = 22 µM ± 3.900 µM (n=3) | EC50 > 50 µM (n=3) |