Potency data for probe BI-5524 and control BI-5525
In vitro potency
| Target name | Target information | Species | Assay description | BI-5524 | BI-5525 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| ELANE | purified commercial protein | Homo sapiens | ELANE inhibitor assay (human): Biochemical assay using purified commercial human ELANE and a fluorescent peptide substrate. | IC50 = 0.5 nM | IC50 = 41 nM | |
| ELANE | Mus musculus | ELANE inhibitor assay (mouse) | IC50 = 12 nM | |||
| ELANE | Cricetulus griseus | ELANE inhibitor assay (hamster) | IC50 = 4 nM | |||
| PRTN3 | Homo sapiens | PRTN3 inhibition assay | IC50 = 367 nM | |||
| CTSG | Homo sapiens | CTSG inhibition assay | IC50 > 10 µM |
In cell potency
| Target name | Target information | Species | Assay description | BI-5524 | BI-5525 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| ELANE | human plasma | Homo sapiens | Inhibition of zymosan-stimulated ELANE activity in human plasma | IC50 = 1.2 nM | IC50 = 492 nM |