Potency data for probe BI 605906 and control BI-5026
In vitro potency
| Target name | Target information | Species | Assay description | BI 605906 | BI-5026 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| IKBKB | Homo sapiens | Biochemical assay | IC50 = 49 nM | IC50 > 10 µM | ||
| AAK1 | Homo sapiens | Dose response measurement (AAK1) | IC50 = 272 nM | |||
| GAK | Homo sapiens | Dose response measurement (GAK) | IC50 = 188 nM | |||
| IRAK3 | Homo sapiens | Dose response measurement (IRAK3) | IC50 = 921 nM |
In cell potency
| Target name | Target information | Species | Assay description | BI 605906 | BI-5026 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| NFKBIA | HeLa cells | Homo sapiens | Inhibition of phospho-IκBα in HeLa cells | EC50 = 0.9 µM | ||
| ICAM1 | HeLa cells | Homo sapiens | Inhibition of expression of ICAM1 in HeLa cells | EC50 = 0.7 µM | ||
| AAK1 | Homo sapiens | NanoBRET (SGC-FFM) (AAK1) | IC50 = 7 µM | |||
| GAK | Homo sapiens | NanoBRET (SGC-FFM) (GAK) | IC50 = 6.5 µM | |||
| IRAK3 | Homo sapiens | NanoBRET (SGC-FFM) (IRAK3) | IC50 > 20 µM |