Potency data for probe CCT369260 and control CCT393732
In vitro potency
| Target name | Target information | Species | Assay description | CCT369260 | CCT393732 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| BCL6 | recombinant protein expressed in E.coli; human BCL6 BTB domain (amino-acids 5-129) with HIS-tag | Homo sapiens | TR-FRET | IC50 = 520 nM (n=11) | IC50 = 21 µM | PMID: 32275432 |
In cell potency
| Target name | Target information | Species | Assay description | CCT369260 | CCT393732 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| BCL6 | OCI-Ly1 cells; endogenous BCL6 | Homo sapiens | MSD (Meso Scale Discovery) degrader assay (OCI-Ly1 cells): 2 hours incubation at 37 °C | DC50 = 49 nM | DC50 = inactive | PMID: 32275432 |
| BCL6 | Karpas 422 cells; endogenous BCL6 | Homo sapiens | MSD (Meso Scale Discovery) degrader assay (Karpas 422 cells) | DC50 = 62 nM | PMID: 32275432 | |
| BCL6 | SUDHL-4 cells; endogenous BCL6 | Homo sapiens | Immunofluorescence-based BCL6 degradation assay in SUDHL-4 cells | DC50 = 90 nM | PMID: 32275432 | |
| BCL6 | SUDHL-4 cells | Homo sapiens | Proliferation assay (SUDHL-4 cells) | GI50 = 92 nM | PMID: 32275432 | |
| BCL6 | OCI-Ly1 cells | Homo sapiens | Proliferation assay (OCI-Ly1 cells) | GI50 = 35 nM | PMID: 32275432 | |
| BCL6 | OCI-Ly3 cells | Homo sapiens | Proliferation assay (OCI-Ly3 cells): low BCL6 expressing cells | GI50 = 1610 nM | PMID: 32275432 |