Potency data for probe Huib32 and control Huib32NC
In vitro potency
| Target name | Target information | Species | Assay description | Huib32 | Huib32NC | Publication of assay conditions |
|---|---|---|---|---|---|---|
| USP32 | recombinant human protein | Homo sapiens | Activity assay using Ub-RhoMP (USP32): Substrate: fluorogenic ubiquitin-rhodamine-morpholine (UbRhoMP) | IC50 = 21.2 nM | IC50 = 6.027 µM | Eurofins assay |
| USP32 | recombinant human protein | Homo sapiens | Activity assay using Ub-RhoMP (USP32, KIapp): Substrate: fluorogenic ubiquitin-rhodamine-morpholine (UbRhoMP) | kinact/KIapp = 12285 M-1s-1 | Eurofins assay |
In cell potency
| Target name | Target information | Species | Assay description | Huib32 | Huib32NC | Publication of assay conditions |
|---|---|---|---|---|---|---|
| USP32 | HEK293T cells | Homo sapiens | ABPP assay (USP32): Activity based protein profiling assay | Max inhibition = 5-10 µM | Eurofins assay |