In cells

Use at a concentration of 5 µM.

No significant toxicity in MelJuSo, HEK293 and HeLa cells incubated with 5 to 10 µM Huib32 for 72h (Cell Titer Blue assay) was observed.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Not tested in vivo.