Potency data for probe JNJ-39758979 and control JNJ-39668551
In vitro potency
| Target name | Target information | Species | Assay description | JNJ-39758979 | JNJ-39668551 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| HRH4 | Membranes from SK-N-MC cells transfected with human H4 receptor | Homo sapiens | Binding assay | Ki = 12.5 nM | Ki > 10 µM | PMID: 14722321 |
In cell potency
| Target name | Target information | Species | Assay description | JNJ-39758979 | JNJ-39668551 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| HRH4 | SK-N-MC cells expressing human H4 receptor | Homo sapiens | Cell based cAMP Assays (pA2) : pA2 indicates the conc. of antagonist when double the agonist is required to have the same effect on the receptor as when no antagonist is present | pA2 = 7.9 | pA2 < 4 | PMID: 14722321 |
| HRH4 | Mouse bone marrow mast cell (mBMMC) | Mus musculus | mBMMC chemotaxis | IC50 = 8 nM | PMID: 14722321 |