Potency data for probe JNJ-4355 and control JNJ-78732576
In vitro potency
| Target name | Target information | Species | Assay description | JNJ-4355 | JNJ-78732576 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| MCL1 | Homo sapiens | HTRF (MCL1): 1XTb-Mcl-1 and Cy5Bim was used.TheTR-FRET signal was read on an BMGPHERAStar FSX MicroPlateReader at roomtemperature usingt he HTRF optic module. | Ki = 0.015 nM | Ki = 115 nM | Slide 7 in JNJ-4355 Inhibitor for MCL1.pdf | |
| BCL2 | Homo sapiens | HTRF (BCL2) | Ki > 3.75 µM | Slide 7 in JNJ-4355 Inhibitor for MCL1.pdf | ||
| BCL2A1 | Homo sapiens | HTRF (BCL2A1) | Ki > 5 µM | Slide 7 in JNJ-4355 Inhibitor for MCL1.pdf | ||
| BCL2L1 | Homo sapiens | HTRF (BCL2L1) | Ki > 5 µM | Slide 7 in JNJ-4355 Inhibitor for MCL1.pdf |
In cell potency
| Target name | Target information | Species | Assay description | JNJ-4355 | JNJ-78732576 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| MCL1 | human MOLP8 cells | Homo sapiens | Caspase Glo MOLP-8 assay: Luminescent assay that measures caspase-3 and -7 activities in purified enzyme preparations or cultures of adherent or suspension cells. | AC50 = 12 nM | AC50 > 30 µM | Slide 8 in JNJ-4355 Inhibitor for MCL1.pdf |