Potency data for probe PFI-653 and control PFI-653-N
In vitro potency
| Target name | Target information | Species | Assay description | PFI-653 | PFI-653-N | Publication of assay conditions |
|---|---|---|---|---|---|---|
| VNN1 | Recombinant human vanin-1 | Homo sapiens | Human recombinant vanin-1 assay : Performed at a vanin-1 concentration of 25 pM | IC50 = 6.85 nM | IC50 = 17.6 µM | |
| VNN1 | Recombinant mouse vanin-1 | Mus musculus | Mouse recombinant vanin-1 assay | IC50 = 24.5 nM | IC50 = 15 µM | |
| BTD | Homo sapiens | Biotinidase assay | IC50 > 50 µM |
In cell potency
| Target name | Target information | Species | Assay description | PFI-653 | PFI-653-N | Publication of assay conditions |
|---|---|---|---|---|---|---|
| VNN1 | Human plasma vanin-1 | Homo sapiens | Human plasma vanin-1 assay: Performed with 8 nM vanin-1 | IC50 = 9 nM | IC50 = 3 µM | |
| VNN1 | Mouse plasma vanin-1 | Mus musculus | Mouse plasma vanin-1 assay | IC50 = 53.4 nM | IC50 = 15 µM | |
| VNN1 | Rat plasma vanin-1 | Rattus sp. | Rat plasma vanin-1 assay | IC50 = 12 nM |