Potency data for probe Skepinone-L and control FM-743
In cell potency
| Target name | Target information | Species | Assay description | Probe result (SD) | Control result (SD) | Publication of assay conditions |
|---|---|---|---|---|---|---|
| MAPK14 | Homo sapiens | Biochemical assay: cell free ELISA | IC50 = 5 nM | PMID: 22198732 | ||
| MAPK14, MAPK14 | HEK cells; full length | Homo sapiens | NanoBRET (MAPK14) | IC50 = 13.600 nM | IC50 = 11.300 µM | PMID: 31702918 |
| MAPK11, MAPK11 | HEK cells | Homo sapiens | NanoBRET (MAPK11) | IC50 = 40.200 nM | IC50 = 16.200 µM | PMID: 31702918 |
| HSPB1 | Anisomycin treated HeLa cells | Homo sapiens | Inhibition of HSP27 (Ser82) (Western blot) | IC50 = 25 nM ± 9.000 nM (n=3) | PMID: 22198732 | |
| TNF | Homo sapiens | TNF-α release ( LPS stim., WB) | IC50 = 22-50 nM | PMID: 22198732 | ||
| TNF | activated hPBMC cells | Homo sapiens | TNF-α and IL-1β and IL-10 release | IC50 = 30-50 nM | PMID: 22198732 |