Potency data for probe T3-CLK and control T3-CLK-N
In vitro potency
| Target name | Target information | Species | Assay description | T3-CLK | T3-CLK-N | Publication of assay conditions |
|---|---|---|---|---|---|---|
| CLK1 | Homo sapiens | In vitro kinase assay (CLK1): 1 mM ATP | IC50 = 0.67 nM | PMID: 28232751 | ||
| CLK2 | Homo sapiens | In vitro kinase assay (CLK2): 1 mM ATP | IC50 = 15 nM | PMID: 28232751 | ||
| CLK3 | Homo sapiens | In vitro kinase assay (CLK3): 1 mM ATP | IC50 = 110 nM | PMID: 28232751 | ||
| DYRK1A | Homo sapiens | In vitro kinase assay (DYRK1A): 1 mM ATP | IC50 = 260 nM | PMID: 28232751 | ||
| DYRK1B | Homo sapiens | In vitro kinase assay (DYRK1B): 1 mM ATP | IC50 = 230 nM | PMID: 28232751 |
In cell potency
| Target name | Target information | Species | Assay description | T3-CLK | T3-CLK-N | Publication of assay conditions |
|---|---|---|---|---|---|---|
| CLK1 | Homo sapiens | NanoBRET (SGC Frankfurt) (CLK1) | IC50 = 3.49 nM | IC50 > 10 µM | ||
| CLK2 | Homo sapiens | NanoBRET (SGC Frankfurt) (CLK2) | IC50 = 16.6 nM | IC50 > 10 µM | ||
| CLK4 | Homo sapiens | NanoBRET (SGC Frankfurt) (CLK4) | IC50 = 1.98 nM | IC50 > 10 µM | ||
| DYRK1A | Homo sapiens | NanoBRET (SGC Frankfurt) (DYRK1A) | IC50 = 32 nM | IC50 > 10 µM | ||
| DYRK1B | Homo sapiens | NanoBRET (SGC Frankfurt) (DYRK1B) | IC50 = 67 nM | IC50 > 10 µM | ||
| SIK2 | Homo sapiens | NanoBRET (SGC Frankfurt) (SIK2): Measured at tracer Kd | IC50 = 125 nM | IC50 > 10 µM | ||
| FLT3 | Homo sapiens | NanoBRET (SGC Frankfurt) (FLT3): Measured at tracer Kd | IC50 = 668 nM | IC50 > 10 µM | ||
| FLT3 (D835V) | Homo sapiens | NanoBRET (SGC Frankfurt) (FLT3 (D835V)): Measured at tracer Kd | IC50 = 138 nM | IC50 > 10 µM |