Potency data for probe TP-030-1 and control TP-030n
In vitro potency
| Target name | Target information | Species | Assay description | TP-030-1 | TP-030n | Publication of assay conditions |
|---|---|---|---|---|---|---|
| RIPK1, RIPK1 | GST-His-RIPK1(1−375): BaculoDirect baculovirus expression system; AA 1-375 | Homo sapiens | TR-FRET (human RIPK1) | Ki = 3.9 nM | Ki = 6900 nM | PMID: 29485864 |
| RIPK1 | Mus musculus | TR-FRET (mouse RIPK1) | IC50 = 4200 nM | IC50 > 10 µM | PMID: 29485864 |
In cell potency
| Target name | Target information | Species | Assay description | TP-030-1 | TP-030n | Publication of assay conditions |
|---|---|---|---|---|---|---|
| human colorectal adenocarcinoma HT-29 cells | Homo sapiens | HT29 necroptosis assay | IC50 = 18 nM | PMID: 29485864 |