In cells

Use at concentration of 100 nM.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Tested in mice with 50 - 200 mg/kg BID. A‐446 showed moderate dose dependent tumor growth inhibition across multiple lung cancer cell lines in mouse xenograft studies.