In cells

Use ABT-724 in cellular assays between 1 nM and 10000 nM. Do not exceed concentrations of 10000 nM to avoid unspecific effects. Use together with negative control at highest dose of probe.

We recommend to use the Dopamine Receptor D4 agonist probe UCSF924 also available from SGC for control experiments.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

In rat ABT-724 showed high stability and dose-dependently facilitates penile erection with a maximal effect at a 0.003 μmol/kg s.c. dose.