In cells

Use at concentrations between at 30 – 500 nM with a maximum concentration of 2.5 µM for experiments with human ALOX5AP. Note: There is a strong species difference. BI 665915 is a much weaker inhibitor for mouse ALOX5AP. Since concentrations of up to 25 µM would be necessary, it is not recommended for the murine system.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Tested in rat, dog, and cynomolgus monkey with 1 mg/kg (iv) and 10 mg/kg (po).