In cells

Use at a concentration of 100 nM.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Tested in rat with 15 -60 µg injected into the paraventricular nucleus (PVN). BIBO3304 is able to reduce food intake of rats that were stimulated either by endogenous (induced by fasting) or by exogenous NPY application.