In cells

Use at concentration up to 10 µM.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Tested in non-tumor-bearing C57Bl/6 mice with an oral administration of up to 30 mg/kg QD for 10 days. It was well tolerated without body weight loss or side effects.
Modulation of total lactate was shown in MC38 tumor-bearing mice (MCT4 high/MCT1 low) if combined with the MCT1/2 inhibitor AZD3965.