In cells

Use at a concentration up to 1 µM.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

Tested in rats (p.o. dose 0.3 to 3 mg/kg) and monkeys. No significant CNS side effects in neurological assessment in rats were observed. Significantly (p<0.01) reversed the scopolamine produced impairment of working memory in the delayed match to position (DMTP) task at 0.3 and 1 mg/kg p.o. No progression to clinic due to liver findings from a 2-weeks DRF (Dose-range finding) tox in rats. Shows autoinduction upon chronic administration in rats due to potent induction of CYP1A2.