Potency data for probe RO-275 and control RO7239047
No in vitro potency data available.
In cell potency
| Target name | Target information | Species | Assay description | RO-275 | RO7239047 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| HCN1 | HCN1 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | Automated patch clamp after a single hyperpolarizing pulse -80 mV (HCN1) | IC50 = 48 nM | PMID: 38042151 | |
| HCN1 | HCN1 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | Automated patch clamp after a single hyperpolarizing pulse -100 mV (HCN1) | IC50 = 46 nM | PMID: 38042151 | |
| HCN1 | HCN1 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | Automated patch clamp after a single hyperpolarizing pulse -120 mV (HCN1) | IC50 = 43 nM | PMID: 38042151 | |
| HCN1 | HCN1 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | Automated patch clamp after 50 hyperpolarizing pulses of -100 mV (HCN1): Simulation of use dependency (UD) | IC50 = 49 nM | PMID: 38042151 | |
| HCN1 | HCN1 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | Electrophysiology recordings (human HCN1): Manual whole-cell patch clamp | IC50 = 269 nM | PMID: 38042151 | |
| HCN1 | rat HCN1 overexpressing HEK293 cells | Rattus norvegicus | Electrophysiology recordings (rat HCN1): Manual whole-cell patch clamp | IC50 = 240 nM | PMID: 38042151 | |
| HCN1, PEX5L | human HCN1 in co-expression with TRIP8b in HEK293 cells | Homo sapiens | Electrophysiology recordings (human HCN1, PEX5L): Manual whole-cell patch clamp | IC50 = 312 nM | PMID: 38042151 | |
| HCN1, HCN2 | hHCN1/ hHCN2 tandem overexpressing HEK293 cells | Homo sapiens | Electrophysiology recordings (human HCN1, HCN2 tandem): Manual whole-cell patch clamp | IC50 = 423 nM | PMID: 38042151 | |
| HCN2 | HCN2 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | Automated patch clamp after a single hyperpolarizing pulse -100 mV (HCN2) | IC50 = 14.3 µM | PMID: 38042151 | |
| HCN3 | HCN3 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | Automated patch clamp after a single hyperpolarizing pulse -100 mV (HCN3) | IC50 = 4.6 µM | PMID: 38042151 | |
| HCN4 | HCN4 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | Automated patch clamp after a single hyperpolarizing pulse -100 mV (HCN4) | IC50 = 13.9 µM | PMID: 38042151 | |
| HCN1 | HCN1 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | MC profiling (-80 mV, HCN1): Combination of patch clamp recordings with multi-electrode array technology | IC50 = 40 µM | ||
| HCN1 | HCN1 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | MC profiling (-100 mV, HCN1): Combination of patch clamp recordings with multi-electrode array technology | IC50 = 40 µM | ||
| HCN1 | HCN1 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | MC profiling (-120 mV, HCN1): Combination of patch clamp recordings with multi-electrode array technology | IC50 = 40 µM | ||
| HCN2 | HCN2 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | MC profiling (-80 mV, HCN2): Combination of patch clamp recordings with multi-electrode array technology | IC50 > 40 µM | ||
| HCN2 | HCN2 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | MC profiling (-100 mV, HCN2): Combination of patch clamp recordings with multi-electrode array technology | IC50 > 40 µM | ||
| HCN2 | HCN2 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | MC profiling (-120 mV, HCN2): Combination of patch clamp recordings with multi-electrode array technology | IC50 > 40 µM | ||
| HCN4 | HCN4 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | MC profiling (-80 mV, HCN4): Combination of patch clamp recordings with multi-electrode array technology | IC50 > 40 µM | ||
| HCN4 | HCN4 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | MC profiling (-100 mV, HCN4): Combination of patch clamp recordings with multi-electrode array technology | IC50 > 40 µM | ||
| HCN4 | HCN4 overexpressing HEK293 cells (tetracycline inducible) | Homo sapiens | MC profiling (-120 mV, HCN4): Combination of patch clamp recordings with multi-electrode array technology | IC50 > 40 µM |