In cells

Use at a concentration up to 100 nM. BAY-3153 has a low solubility but a high permeability. It is stable for 4 hours.

We recommend to use the CCR1 antagonist probe BI 639667 also available from SGC for control experiments.

Use the recommended concentration to avoid off-target toxicity and/or off-target activity. Using concentrations that are higher than recommended could lead to false positive or confounding results.

In vivo

The compound is well suited to perform in-vivo studies. It was tested in rats with 10 mg/kg and resulted in a reduction of infiltrating macrophages after renal ischemia/reperfusion injury (I/RI).