Potency data for probe JNJ-6204 and control JNJ-0293
In vitro potency
| Target name | Target information | Species | Assay description | JNJ-6204 | JNJ-0293 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| CSNK1D | purified enzyme | Homo sapiens | ADP-Glo | IC50 = 2.3 nM | IC50 = 6.9 µM | PMID: 19458106 |
| CSNK1E | purified enzyme | Homo sapiens | ADP-Glo | IC50 = 137 nM | PMID: 19458107 |
In cell potency
| Target name | Target information | Species | Assay description | JNJ-6204 | JNJ-0293 | Publication of assay conditions |
|---|---|---|---|---|---|---|
| CSNK1D | U2OS cells transfected with the PER2 gene coupled to a GFP reporter | Homo sapiens | PER2 translocation whole cell functional assay: Measures translocation of PER2::GFP into the nucleus | IC50 = 542 nM | ||
| CSNK1D | CHO cells expressing nanoluciferase-tagged CSNK1D; full length human CSNK1D fused to nanoluciferase | Homo sapiens | BRET whole cell binding assay: Measures bioluminescent fluorescence transfer | IC50 = 72 nM | IC50 > 10 µM |